There are three tests for the obesity virus, each with benefits and drawbacks and they are different in cost. At this time all have to be done by a physician or laboratory to get a sample to send to Obetech.

ADV-36 TESTING

ELISA Test:

The test measures antibodies against the obesity virus in blood. It is a very sensitive screening test, but rarely an incorrect reading may occur. If the ELISA test is positive or if the individual wants a more specific test, the PCR test or the serum neutralization test may be considered.

How it’s done: This test requires a tube of blood be sent to Obetech (10 ml or about two teaspoons of blood)

Cost – Contact Obetech for quote

 

Serum Neutralization Test:

The serum neutralization test is not quite as sensitive as the ELISA, but is more specific. It measures antibodies against the obesity virus in blood. It is a very time consuming and an expensive tissue culture test requiring cells exposed to the obesity virus be examined under the microscope to determine if antibodies in the person’s serum can stop the virus from killing the cells. It has been a “gold standard” test. If it is positive, the person has been infected with the obesity virus.

How it’s done: This test requires a tube of blood be sent to Obetech (10 ml or about two teaspoons of blood) by patient’s doctors or laboratories. It takes two weeks to perform.

Cost – Contact Obetech for quote

 

Polymerase Chain Reaction (PCR) Test:

The PCR test is not quite as sensitive as the ELISA, but it measures Adv36 DNA in fat tissue. If positive, it is very strong evidence that the person has been infected. It looks for the presence of DNA from the obesity virus, not the person’s DNA, and can detect tiny quantities. It is a “gold standard” test. If it is positive, it is virtually certain that the person has been infected with the obesity virus.

Cost – Contact Obetech for quote

 

 OTHER TESTS INCLUDE:

1. Molecular cloning service:

Assist investigators with plasmid manipulations and cloning projects.  The services include:

  1. Endotoxin-free Plasmid purification, insert purification, plasmid management.
  2. Other projects are designed upon the specific needs of client including custom subcloning, library screening etc.

2. Protein expression and purification service:

Assist investigators with protein expression and purification. The services include:

  1. Generation of protein expression vectors.
  2. Recombinant protein expression in bacterial cells (multiple systems).

3. Gene expression analysis:

Assist investigators with comprehensive gene expression analysis.  The services include:

  1. RNA isolation from tissue or mammalian cell lines.
  2. Real time- RT PCR analysis looking for differential gene expression at RNA level.
  3. Immunological blotting looking for differential gene expression at protein level.

4. Tissue culturing:

Assist investigators with tissue culturing and maintaining cell line.  The services include:

  1. Generation of various human primary cell line.
  2. Culturing and maintaining various mammalian cell lines.